亚精胺对黄瓜幼苗灰霉病的诱抗作用
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Û uF 9, 3Q¯, Ä Øl u20é.46 °Û¼ ì»2ËZ H,¨ÿ |Spdÿ ÆÉ öÔÿ2 cmÉô Sº, Äé¨ ¹20 ml,Ø HSpd ·Aà 1n;2dª ë ¨ Éĺ, Ä Øl u¨ ¹60ml, ë、 ¨ ÒÀ ·A¸¿ ë/¯.v¨© £}9.1.3 h ¤Õ410 °, Sºª4d¤Õç ¾h .sY¨ f¹0ÇA ÕZE¤Õ.f¤Õ¨u d u |°å¹0.4cm¥ f¤Õ¿»2 Ë ë, Ä ËP· öò¤Õ1ñ f.¨¿©çhê°å,9 Øh f· .¹0ÇA¤Õ ¥Å¹0ÇA i¹4.3105ñ·ml-1.¨ ¸ 9 |¹0ÇA,|±é»2 Ë Ï À¿¹0ÇAÏÿ5 s, TVñÏ]¸¹0ÇAÐ ð ˤ.¨¿¼ ì ÿ·S 3 Ø 3Ä·S©ç.f¹0ÇA¤Õ] HÉ.¤Õ8ªì5Â{©É Ä5 Ø,æ21±2℃/15±1℃, b A90[ .1.4 ©çZE1.4.1h f· sY¿¤Õª2、4、6 d¨ E3E©çhêå、å, Ü ´T¹hê°å,h f· 9 Ø T Â/h f· ∑ Ø òhê°å Ø ¥hê îµ ØÏKvhê°å1001.4.2 3 Ø 3Ä·S sY¿ºª3、6、9、1215 dg |¼ ì»2 ËÉ 3 Ø 3Ä·S©ç.vMoerschbacher©[ 14]ZE©çPAL、POD;vMiler©[15]ZE©çPPO;vë¡o©[ 16]ZE©ç9p、 ËÛ[c ;vMorison[ 17]ZE©ç É Íc .1.4.3 ÿ·S 426 °, Sºª20d,Ø°¼ ìéÚ、ÔY、 Ë 、 ë、 é7É éÄÉ . ì· ÔY/éÚ éÄÉ 100.îµ ¨SAS ÈqDuncan¯µEÉ1 µsA÷s,mV ¹3Q¯¥ Ü ´.2 ²TÐs2.1 ÷SpdÛ¼ ìh f· ¥Y®m1 V©,ÐvM1,÷Spd üA® m1 ÷SpdÛ¼ ìh f· ¥YFig.1 Effectsofexogenousspermidineondiseaseindexincu-cumberseedlings.Ⅰ0mg·L-1Spd;Ⅱ50mg·L-1 Spd;Ⅲ100 mg·L-1 Spd;Ⅳ150mg·L-1 Spd;Ⅴ200mg·L-1 Spd;Ⅵ250 mg·L-1Spd.826 ¨ 3 ÿ Ð 19 Û¼ ì¥h f· .]©ç H ù¤Õª2、4、6d,Spd iÐh f· ïCM]¥MÄ p, ÛSpd iïv 6Ú,h f· ïv®,®Bçñª,» Û“Spd i¥ 6Ú79F. ª üSpd®Û¼ ìh f· i aR¥ Ø i,[150mg·L-1 ØK®,¤Õª2、4、6 dh f· sY1v® 10.0、18.525.4.2.2 ÷Spdç ¾h S /Û Ë]Þ ½¥Y®V1 V©, Û Sºª HW¥üÉ,ò Ø¥ Sºª6、9 d,POD ÛSpd i¥ 6Úï5®ª 6Ú¥MÄ t ]; Sºª15 d, ÛSpd i¥ 6Ú,PODï5 6Úª®¥MÄ t ].]©ç H ù,Spdò i Ø¥PPOÚ¿v,SpdPPO¥9 9VCBç¥ ir. Sºª3、6 d,Spd i®¿150mg·L-1 H, PPO ÛSpd i¥ 6Ú79 ;Spd iÚ¿150 mg·L-1 H,PPO ÛSpd i¥ 6Ú7h Ð;Spd i¹150 mg·L-1 H,PPOrKv´,sY1v4Ú 15.527.5. Sºª9、12、15 d,PPOÚC200mg·L-1 Ø,sY1v9F 38.9、22.629.7.2.3 ÷Spdç ¾h S /Û ËÏ FhM1þÉc ¥Y®V2 V©, Û Sºª HW¥üÉ,òØ9pc ï5®ª 6Ú ª û Ú £ Ü¥MÄ t ].¤Õç ¾h - Pºª3 d,Spdòi Ø¥9pc Ú¿v,9pc ÛSpdi¥ 6Ú79F,Spd i¹200mg·L-1 HrKv´,1v9F13.7;¤Õç ¾h ª Pºª6、9、12、15 d,Spdò i Ø¥9pc ïv 6Ú,°ÀÚ¿v.V1 ÷Spdç ¾h S /Û¼ ì ËÏ]Þ ½¥YTab.1 EffectsofexogenousspermidineonactivitiesofdefenseenzymesincucumberseedlingsleavesunderinfectionofB.cinereaSpd iSpermdineconcentrationmg·L-1 Sºª9 d,Spdò i Ø¥ ËÛ[c ÐvíA÷µs; Sºª12、15 d,Spdò i Ø¥ ËÛ[c ®¿v.]©ç H ù,Spdò i Ø¥ É Íc Ú¿v,Spd É Íî¥\ÉT¨VCBç¥ ir. Sºª3、6、9 d,Spd i®¿150 mg·L-1 H, É Íc ÛSpd i¥ 6Ú79F,Spd iÚ¿150 mg·L-1 H, É Íc ÛSpd i¥ 6Ú7h ,Spd i¹150 mg·L-1 H, É Íc rKv´,sY1v4Ú 17.8、52.023.6; Sºª12、15 d, É Íc ´C200 mg·L-1 Ø,sY1v9F18.344.5.2.4 ÷Spdç ¾h S /Û¼ ì 3Éâ¥Y®V3 V©,÷Spdç ¾h S /Û¼ ì¥ÔY、éÚ、9 ë、 é7É 、 éÄÉ、 ì· 6[·S µ üA¥\ÉT¨;Z 7 9µ\ÉT¨,ÐvM1µsA÷.SpdÛ¼ ì 3É¥\ÉT¨VC üA¥ ir, Û i¥ïv 6Ú,Spd¼ ì¥\ÉT¨ïv9 ,ò[·SrKv´ª, ÛSpd i¥Ã 6Ú,\ÉT¨ïvh Ð.100 mg·L-1Spd ØÛ¼ ì 3É¥\ÉT¨Kv,ÔY、9 ë、 éÄÉ 、 ì· sY1v4Ú 8.3、35.2、49.432.5.3 “ -,1¿÷Spdº±þ Fh[\ɱé 3É¥ùî7n¡.bÆ©[ 18]ùîV ü,d IâHq/õ¤ ¨÷Spd Vr ìrT. k²TV ü,÷Spd VºÛ¼ ìç¾h¥ F,h f· 1vKÚ®25.4;\É ç ¾h S /Û¼ ì¥ 3Éâ,ÐvM1, ì· KÚ9F32.5.PAL、POD、PPO ±þ Fh}VñÏ¥1o½. ñ ̥ڮбþ FhQµ“ Es á M¥1“,YȨT ±þ8 ]ÏQ¥1·S.PAL 6BZ ë, ñ» ÐXµ¥]Þ ½s0²,Y ²,V7Ø« ½. ùî²TV ü,÷Spd تۼ ì ËÏPAL、POD、PPOïC]¥MÄ t ], Û Sº HW¥üÉ,PALïv®,PODïv9 ,PPOï5®ª 6Ú®¥MÄ t ].ÕMÄ t ]Ðv HW ûBÁ,Spd Ø¥3Õ ½ñ k ùWB°Ú¿v £ Ü,PAL、POD、PPO3Õ]Þ ½KÚsY1v9F48.4、635.938.9.yN,t ½Ð÷SpdºÛ¼ ì Fç ¾hµBç¥M1,Û V YV4Ú828 ¨ 3 ÿ Ð 19 t ½¥9 ·Þhð S Æ、 ¢ü¥ ï.p ËþÉ }Áþ ±þ .¥ Fhy0, “·Þhðþ S ¥T¨, ª¹ ±þ FhÅB[ 22].9p ËÛ[û p ËþÉ, }ÐPAL、POD、PPO á MM1. É Í ±þC¥ö1îsB,Çhð± 3þ S] àpT¨,7 O É Í®s0 p Ë -8[ T¨Á3¥³ Ö V[Ä ¥ 、 ½ý Í[23] . k²TV ü,¤Õç ¾h - Pºª3 d,Spd\É 9p、 ËÛ[¥î};¤Õç ¾h ª, - ù Pºª6 dSpd\É ËÛ[¥î},7]Å 9p¥î},ª ù Pºª12、15 dSpd\É 9p¥î},7]Å ËÛ[¥î}.ñ k ùW,Spd\É ÉÍ¥î}. ª ü÷Spd V YVØ«Û¼ì8 9p、 ËÛ[、 É Í¥} 4Ú ç ¾h¥ F.N, kÏòÕ]Þ ½ FhM1þÉc ¥MÄ t ]VCiBÁ, ½ FhM1þÉc ÚC¥ HW iµs, V ®¿÷Spd  F|.¾ HW ¨½Ð ¥µs ¥, s0 صÉBùî.Bî ª¹,ÂT¹» Pбþ¥ Iâ} ç|Q[ 24-25] .ùîV ü,ÂÄ ½PAOs³Â HÁ 3¥H2O2 V ÇÐCvs0¥ ó Fhµ1,ÎÐ Ñ ùQHR“d¤ FSAR¥|.¾Vñ[ 26].Shen©[ 27]C®Ñ} ç/,Spdç Ø VE¸± ì8ÏNADPHÄ ½¥4Ú,V7]Å Ï} 纥H2O2c 6Ú,4ÚÛ 0 Ï. 6, £ÖSAº¥õ¤ Ëâ ½ å º¥â ½¥ 6Úü YVSpmº¥[ 28]. sC.gloeosporioiddesË ª,Â Ø V[A÷®¾h𥸓 s 3¹0¥î,i]Å Í.Õ]Årö1 ®¿Â]Å Ca2/CaMG |.¾Vñ[ 29].Kasukabe©[ 30]C, Æ Spdî ½cDNA¥ E 2·±é ËÏ,Spdî ½Spdc v üA9F,] H9 Ï、ò、÷、Äl、 1i} çä¤ jý]©} ç¥ F.cDNA± sV ü, Iâ} ç/,y±éÏBtyv c, } çY cy0 ÂDREB} ç ¹â Ârd29A.V üSpd VT¹} ç|. {oåÏ¥|Ø«y0,y±þ} 祷 FÅ. 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